Chromatography calculation directory

HPLC system suitability calculations

Open one calculation at a time. Each page defines its inputs, formula and measurement basis so unrelated fields do not appear together.

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Separate calculations

Calculate the mean, sample standard deviation and relative standard deviation for replicate peak responses.

Method sensitivity

Calculate plate count from retention time and a clearly selected peak-width basis.

Column efficiency

Calculate separation between two adjacent peaks using half-height or baseline widths.

Peak separation

Calculate the USP tailing factor from measurements taken at five percent of peak height.

Peak symmetry